Human IL-6 PicoKine® Quick ELISA Kit

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF) . Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 11 10 13 12 0.99 8.3% Sample 2 42 45 38 40 41 1.96 5.1% Sample 3 134 145 151 147 144 3.6 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 11 10 13 12 0.99 8.3% Sample 1 15 11 10 13 12 0.99 8.3% Sample 2 42 45 38 40 41 1.96 5.1% Sample 2 42 45 38 40 41 1.96 5.1% Sample 3 134 145 151 147 144 3.6 2.5% Sample 3 134 145 151 147 144 3.6 2.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 42 134 13 46 1307 Standard deviation 0.88 2.26 10.31 1.00 2.57 11.05 CV (%) 5.9% 5.4% 7.7% 7.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 42 134 13 46 1307 Standard deviation 0.88 2.26 10.31 1.00 2.57 11.05 CV (%) 5.9% 5.4% 7.7% 7.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 42 134 13 46 1307 Mean (pg/ml) 15 42 134 13 46 1307 Standard deviation 0.88 2.26 10.31 1.00 2.57 11.05 Standard deviation 0.88 2.26 10.31 1.00 2.57 11.05 CV (%) 5.9% 5.4% 7.7% 7.5% 5.6% 8.5% CV (%) 5.9% 5.4% 7.7% 7.5% 5.6% 8.5%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.006 0.081 0.145 0.276 0.486 0.952 1.513 2.074 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.006 0.081 0.145 0.276 0.486 0.952 1.513 2.074 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 Concentration (pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.006 0.081 0.145 0.276 0.486 0.952 1.513 2.074 O.D. 0.006 0.081 0.145 0.276 0.486 0.952 1.513 2.074

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.