Human Lipocalin-2/NGAL PicoKine® Quick ELISA Kit

Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 255 236 267 253 15.43 6.1% Sample 2 1624 1649 1587 1810 1667 120.02 7.2% Sample 3 5541 5497 5323 4757 5279 306.18 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 255 236 267 253 15.43 6.1% Sample 1 285 255 236 267 253 15.43 6.1% Sample 2 1624 1649 1587 1810 1667 120.02 7.2% Sample 2 1624 1649 1587 1810 1667 120.02 7.2% Sample 3 5541 5497 5323 4757 5279 306.18 5.8% Sample 3 5541 5497 5323 4757 5279 306.18 5.8%

Lipocalin-2/NGAL|24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3) ; lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1624 5541 290 1737 5384 Standard deviation 18.52 86.07 243.8 22.04 99 285.35 CV (%) 6.5% 5.3% 4.4% 7.6% 5.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1624 5541 290 1737 5384 Standard deviation 18.52 86.07 243.8 22.04 99 285.35 CV (%) 6.5% 5.3% 4.4% 7.6% 5.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 1624 5541 290 1737 5384 Mean (pg/ml) 285 1624 5541 290 1737 5384 Standard deviation 18.52 86.07 243.8 22.04 99 285.35 Standard deviation 18.52 86.07 243.8 22.04 99 285.35 CV (%) 6.5% 5.3% 4.4% 7.6% 5.7% 5.3% CV (%) 6.5% 5.3% 4.4% 7.6% 5.7% 5.3%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.194 0.330 0.588 1.035 1.606 2.212 2.599 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.194 0.330 0.588 1.035 1.606 2.212 2.599 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.194 0.330 0.588 1.035 1.606 2.212 2.599 O.D. 0.030 0.194 0.330 0.588 1.035 1.606 2.212 2.599

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.