Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte- macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Sample 3 314 297 303 287 300 8.1 2.7%

TNF-alpha|APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2) ; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.