Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit

Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte- macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Sample 3 314 297 303 287 300 8.1 2.7%

Synonyms

TNF-alpha|APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2) ; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Gene Name

CST3

Gene ID

21926

UniProt

P06804

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Capture Antibody

Monoclonal antibody from hamster

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL (cell culture supernatants), 7.8 pg/mL - 500 pg/mL (mouse serum, plasma)

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2%

Sensitivity

<1 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Tnf

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor

Protein Name

Tumor necrosis factor

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.