Human TIMP3 PicoKine® Quick ELISA Kit

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 257 260 256 250 11.25 4.5% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 3 4394 4601 4288 4589 4468 285.95 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 257 260 256 250 11.25 4.5% Sample 1 228 257 260 256 250 11.25 4.5% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 3 4394 4601 4288 4589 4468 285.95 6.4% Sample 3 4394 4601 4288 4589 4468 285.95 6.4%

TIMP-3|HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory) ; SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.