Human TIMP3 PicoKine® Quick ELISA Kit

Quick ELISA Kit

Product Specifications

Background

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 257 260 256 250 11.25 4.5% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 3 4394 4601 4288 4589 4468 285.95 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 257 260 256 250 11.25 4.5% Sample 1 228 257 260 256 250 11.25 4.5% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 2 1364 1309 1336 1522 1382 81.53 5.9% Sample 3 4394 4601 4288 4589 4468 285.95 6.4% Sample 3 4394 4601 4288 4589 4468 285.95 6.4%

Synonyms

TIMP-3|HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory) ; SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3

Gene Name

CST3

Gene ID

7078

UniProt

P35625

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C24-P211

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin , EDTA) and saliva

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1364 4394 244 1487 4066 Mean (pg/ml) 228 1364 4394 244 1487 4066 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 Standard deviation 14.59 90.02 237.27 19.03 107.06 333.41 CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2% CV (%) 6.4% 6.6% 5.4% 7.8% 7.2% 8.2%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human TIMP3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

TIMP metallopeptidase inhibitor 3

Protein Name

Metalloproteinase inhibitor 3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388 O.D. 0.111 0.160 0.190 0.231 0.414 1.003 2.177 2.388

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.