Rat BDNF PicoKine® Quick ELISA Kit

Rat BDNF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF) . BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP) . BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 98 94 105 98 6.17 6.3% Sample 2 302 286 265 296 287 12.34 4.3% Sample 3 945 983 914 962 951 46.59 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 98 94 105 98 6.17 6.3% Sample 1 95 98 94 105 98 6.17 6.3% Sample 2 302 286 265 296 287 12.34 4.3% Sample 2 302 286 265 296 287 12.34 4.3% Sample 3 945 983 914 962 951 46.59 4.9% Sample 3 945 983 914 962 951 46.59 4.9%

Synonyms

Bdnf; Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Gene Name

CST3

Gene ID

24225

UniProt

P23363

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H129-R247

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Monoclonal antibody from mouse

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 302 947 88 289 1024 Standard deviation 4.94 16.91 72.91 4.75 21.67 86.01 CV (%) 5.2% 5.6% 7.7% 5.4% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 302 947 88 289 1024 Standard deviation 4.94 16.91 72.91 4.75 21.67 86.01 CV (%) 5.2% 5.6% 7.7% 5.4% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 302 947 88 289 1024 Mean (pg/ml) 95 302 947 88 289 1024 Standard deviation 4.94 16.91 72.91 4.75 21.67 86.01 Standard deviation 4.94 16.91 72.91 4.75 21.67 86.01 CV (%) 5.2% 5.6% 7.7% 5.4% 7.5% 8.4% CV (%) 5.2% 5.6% 7.7% 5.4% 7.5% 8.4%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Rat Bdnf

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Brain-derived neurotrophic factor

Protein Name

Brain-derived neurotrophic factor

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.