Human BDNF PicoKine® Quick ELISA Kit

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF) . BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP) . BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 55 52 52 52 2.7 5.2% Sample 2 234 198 232 224 169 11.32 6.7% Sample 3 1124 1121 884 1142 1067 76.82 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 55 52 52 52 2.7 5.2% Sample 1 50 55 52 52 52 2.7 5.2% Sample 2 234 198 232 224 169 11.32 6.7% Sample 2 234 198 232 224 169 11.32 6.7% Sample 3 1124 1121 884 1142 1067 76.82 7.2% Sample 3 1124 1121 884 1142 1067 76.82 7.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 234 1124 48 254 987 Standard deviation 3.6 11.23 55.07 4.17 13.97 63.16 CV (%) 7.2% 4.8% 4.9% 8.7% 5.5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 234 1124 48 254 987 Standard deviation 3.6 11.23 55.07 4.17 13.97 63.16 CV (%) 7.2% 4.8% 4.9% 8.7% 5.5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 234 1124 48 254 987 Mean (pg/ml) 50 234 1124 48 254 987 Standard deviation 3.6 11.23 55.07 4.17 13.97 63.16 Standard deviation 3.6 11.23 55.07 4.17 13.97 63.16 CV (%) 7.2% 4.8% 4.9% 8.7% 5.5% 6.4% CV (%) 7.2% 4.8% 4.9% 8.7% 5.5% 6.4%

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955 O.D. 0.014 0.039 0.076 0.120 0.255 0.485 1.020 1.955

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.