Mouse IL-6/Interleukin-6 PicoKine® Quick ELISA Kit

Mouse IL-6/Interleukin-6 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF) . Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 41 45 50 44 2.42 5.5% Sample 2 168 157 157 171 163 9.61 5.9% Sample 3 445 452 467 436 450 24.3 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 41 45 50 44 2.42 5.5% Sample 1 40 41 45 50 44 2.42 5.5% Sample 2 168 157 157 171 163 9.61 5.9% Sample 2 168 157 157 171 163 9.61 5.9% Sample 3 445 452 467 436 450 24.3 5.4% Sample 3 445 452 467 436 450 24.3 5.4%

Synonyms

Interleukin-6; Il6

Gene Name

CST3

Gene ID

16193

UniProt

P08505

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-T211

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 168 445 41 172 459 Standard deviation 2.16 10.24 24.47 3.15 13.41 29.83 CV (%) 5.4% 6.1% 5.5% 7.7% 7.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 168 445 41 172 459 Standard deviation 2.16 10.24 24.47 3.15 13.41 29.83 CV (%) 5.4% 6.1% 5.5% 7.7% 7.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 168 445 41 172 459 Mean (pg/ml) 40 168 445 41 172 459 Standard deviation 2.16 10.24 24.47 3.15 13.41 29.83 Standard deviation 2.16 10.24 24.47 3.15 13.41 29.83 CV (%) 5.4% 6.1% 5.5% 7.7% 7.8% 6.5% CV (%) 5.4% 6.1% 5.5% 7.7% 7.8% 6.5%

Sensitivity

<1 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Il6

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin 6

Protein Name

Interleukin-6

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.055 0.110 0.183 0.317 0.588 1.068 1.829 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.055 0.110 0.183 0.317 0.588 1.068 1.829 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.055 0.110 0.183 0.317 0.588 1.068 1.829 O.D. 0.022 0.055 0.110 0.183 0.317 0.588 1.068 1.829

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.