Human G-CSF PicoKine® Quick ELISA Kit

Human G-CSF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 55 63 58 60 2.52 4.2% Sample 2 240 210 242 227 229 11.45 5% Sample 3 981 965 892 988 956 43.02 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 55 63 58 60 2.52 4.2% Sample 1 65 55 63 58 60 2.52 4.2% Sample 2 240 210 242 227 229 11.45 5% Sample 2 240 210 242 227 229 11.45 5% Sample 3 981 965 892 988 956 43.02 4.5% Sample 3 981 965 892 988 956 43.02 4.5%

Synonyms

G-CSF; Pluripoietin; Filgrastim; Lenograstim

Gene Name

CST3

Gene ID

1440

UniProt

P09919

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T31-P204

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 240 981 67 233 946 Standard deviation 3.96 14.88 43.16 5.15 18.17 61.49 CV (%) 6.1% 6.2% 4.4% 7.7% 7.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 240 981 67 233 946 Standard deviation 3.96 14.88 43.16 5.15 18.17 61.49 CV (%) 6.1% 6.2% 4.4% 7.7% 7.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 65 240 981 67 233 946 Mean (pg/ml) 65 240 981 67 233 946 Standard deviation 3.96 14.88 43.16 5.15 18.17 61.49 Standard deviation 3.96 14.88 43.16 5.15 18.17 61.49 CV (%) 6.1% 6.2% 4.4% 7.7% 7.8% 6.5% CV (%) 6.1% 6.2% 4.4% 7.7% 7.8% 6.5%

Sensitivity

<4 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CSF3

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Colony stimulating factor 3

Protein Name

Granulocyte colony-stimulating factor

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.058 0.092 0.170 0.319 0.599 1.080 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.058 0.092 0.170 0.319 0.599 1.080 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.058 0.092 0.170 0.319 0.599 1.080 1.902 O.D. 0.002 0.058 0.092 0.170 0.319 0.599 1.080 1.902

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.