Human Cystatin C/CST3 PicoKine® Quick ELISA Kit

Human Cystatin C/CST3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes) . Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 695 651 676 688 677 14.89 2.2% Sample 2 2907 2015 2878 2769 2642 73.97 2.8% Sample 3 11478 9967 8907 10146 10124 546.69 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 695 651 676 688 677 14.89 2.2% Sample 1 695 651 676 688 677 14.89 2.2% Sample 2 2907 2015 2878 2769 2642 73.97 2.8% Sample 2 2907 2015 2878 2769 2642 73.97 2.8% Sample 3 11478 9967 8907 10146 10124 546.69 5.4% Sample 3 11478 9967 8907 10146 10124 546.69 5.4%

Synonyms

Cystatin-C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin; CST3

Gene Name

CST3

Gene ID

1471

UniProt

P01034

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A146

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) . Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Applications

ELISA

Field of Research

Apoptosis, Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Intracellular, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 695 2907 11478 712 3114 12116 Standard deviation 45.87 212.21 791.98 56.24 255.34 1054.09 CV (%) 6.6% 7.3% 6.9% 7.9% 8.2% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 695 2907 11478 712 3114 12116 Standard deviation 45.87 212.21 791.98 56.24 255.34 1054.09 CV (%) 6.6% 7.3% 6.9% 7.9% 8.2% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 695 2907 11478 712 3114 12116 Mean (pg/ml) 695 2907 11478 712 3114 12116 Standard deviation 45.87 212.21 791.98 56.24 255.34 1054.09 Standard deviation 45.87 212.21 791.98 56.24 255.34 1054.09 CV (%) 6.6% 7.3% 6.9% 7.9% 8.2% 8.7% CV (%) 6.6% 7.3% 6.9% 7.9% 8.2% 8.7%

Sensitivity

<10 pg/mL

Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

15799 MW

Specificity

Natural and recombinant Human CST3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cystatin-C

Subcellular Location

Secreted .

Protein Name

Cystatin-C

Sample Data

Concentration (pg/ml) 0 312.5 625 1250 2500 5000 10000 20000 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 Concentration (pg/ml) 0 312.5 625 1250 2500 5000 10000 20000 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 Concentration (pg/ml) 0 312.5 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312.5 625 1250 2500 5000 10000 20000 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.