Human CD21/CR2 PicoKine® Quick ELISA Kit

Human CD21/CR2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1242 1468 1273 1362 1336 87.89 6.5% Sample 2 7916 7621 6548 6832 7229 558.34 7.7% Sample 3 24012 23247 26467 22418 24036 1512.51 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1242 1468 1273 1362 1336 87.89 6.5% Sample 1 1242 1468 1273 1362 1336 87.89 6.5% Sample 2 7916 7621 6548 6832 7229 558.34 7.7% Sample 2 7916 7621 6548 6832 7229 558.34 7.7% Sample 3 24012 23247 26467 22418 24036 1512.51 6.2% Sample 3 24012 23247 26467 22418 24036 1512.51 6.2%

Synonyms

Complement receptor type 2; Cr2; Complement C3d receptor; Epstein-Barr virus receptor; EBV receptor; CD21; CR2; C3DR

Gene Name

CR2

Gene ID

1380

UniProt

P20023

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Ile21-71

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 260 913 58 268 861 Standard deviation 3.36 12.22 49.30 4.40 13.93 55.96 CV (%) 5.7% 4.7% 5.4% 7.6% 5.2% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 260 913 58 268 861 Standard deviation 3.36 12.22 49.30 4.40 13.93 55.96 CV (%) 5.7% 4.7% 5.4% 7.6% 5.2% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 260 913 58 268 861 Mean (pg/ml) 59 260 913 58 268 861 Standard deviation 3.36 12.22 49.30 4.40 13.93 55.96 Standard deviation 3.36 12.22 49.30 4.40 13.93 55.96 CV (%) 5.7% 4.7% 5.4% 7.6% 5.2% 6.5% CV (%) 5.7% 4.7% 5.4% 7.6% 5.2% 6.5%

Sensitivity

<20 pg/mL

Function

Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

112916 MW

Specificity

Natural and recombinant human CD21

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Complement receptor type 2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the receptors of complement activation (RCA) family.

Protein Name

Complement receptor type 2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.014 0.096 0.168 0.317 0.565 0.938 1.757 2.382 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.014 0.096 0.168 0.317 0.565 0.938 1.757 2.382 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.014 0.096 0.168 0.317 0.565 0.938 1.757 2.382 O.D. 0.014 0.096 0.168 0.317 0.565 0.938 1.757 2.382

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.