Human Serpin C1/Antithrombin-III PicoKine® Quick ELISA Kit

Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7) . Antithrombin III regulates clot formation both by inhibiting thrombin activity ly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 647 649 673 636 651 13.49 2% Sample 2 1973 1798 1681 1827 1819 103.99 5.7% Sample 3 8982 8247 8966 9377 8893 407.68 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 647 649 673 636 651 13.49 2% Sample 1 647 649 673 636 651 13.49 2% Sample 2 1973 1798 1681 1827 1819 103.99 5.7% Sample 2 1973 1798 1681 1827 1819 103.99 5.7% Sample 3 8982 8247 8966 9377 8893 407.68 4.5% Sample 3 8982 8247 8966 9377 8893 407.68 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 647 1973 8982 647 2050 9365 Standard deviation 28.46 153.89 377.24 34.29 174.25 552.53 CV (%) 4.4% 7.8% 4.2% 5.3% 8.5% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 647 1973 8982 647 2050 9365 Standard deviation 28.46 153.89 377.24 34.29 174.25 552.53 CV (%) 4.4% 7.8% 4.2% 5.3% 8.5% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 647 1973 8982 647 2050 9365 Mean (pg/ml) 647 1973 8982 647 2050 9365 Standard deviation 28.46 153.89 377.24 34.29 174.25 552.53 Standard deviation 28.46 153.89 377.24 34.29 174.25 552.53 CV (%) 4.4% 7.8% 4.2% 5.3% 8.5% 5.9% CV (%) 4.4% 7.8% 4.2% 5.3% 8.5% 5.9%

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.011 0.062 0.082 0.15 0.295 0.582 1.333 1.974 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.011 0.062 0.082 0.15 0.295 0.582 1.333 1.974 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.011 0.062 0.082 0.15 0.295 0.582 1.333 1.974 O.D. 0.011 0.062 0.082 0.15 0.295 0.582 1.333 1.974

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.