Mouse RANK / CD265 PicoKine® Quick ELISA Kit

Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III) . RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 216 198 186 202 11.52 5.7% Sample 2 719 685 707 666 694 20.36 2.9% Sample 3 1032 1046 1090 1088 1064 25.49 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 216 198 186 202 11.52 5.7% Sample 1 210 216 198 186 202 11.52 5.7% Sample 2 719 685 707 666 694 20.36 2.9% Sample 2 719 685 707 666 694 20.36 2.9% Sample 3 1032 1046 1090 1088 1064 25.49 2.3% Sample 3 1032 1046 1090 1088 1064 25.49 2.3%

Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; CD265; Tnfrsf11a; Rank

Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 719 1032 218 725 978 Standard deviation 11.97 30.19 46.44 15.8% 42.05 53.79 CV (%) 5.7% 4.2% 4.5% 7.5% 5.8% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 719 1032 218 725 978 Standard deviation 11.97 30.19 46.44 15.8% 42.05 53.79 CV (%) 5.7% 4.2% 4.5% 7.5% 5.8% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 719 1032 218 725 978 Mean (pg/ml) 210 719 1032 218 725 978 Standard deviation 11.97 30.19 46.44 15.8% 42.05 53.79 Standard deviation 11.97 30.19 46.44 15.8% 42.05 53.79 CV (%) 5.7% 4.2% 4.5% 7.5% 5.8% 5.5% CV (%) 5.7% 4.2% 4.5% 7.5% 5.8% 5.5%

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.109 0.205 0.432 0.719 1.184 1.659 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.109 0.205 0.432 0.719 1.184 1.659 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.109 0.205 0.432 0.719 1.184 1.659 2.038 O.D. 0.006 0.109 0.205 0.432 0.719 1.184 1.659 2.038

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.