Human GDF-15 PicoKine® Quick ELISA Kit

GDF-15 (Growth differentiation factor 15), also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 56 49 57 53 3.11 5.8% Sample 2 252 245 249 271 254 9.98 3.9% Sample 3 1068 1033 966 984 1012 40.23 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 56 49 57 53 3.11 5.8% Sample 1 53 56 49 57 53 3.11 5.8% Sample 2 252 245 249 271 254 9.98 3.9% Sample 2 252 245 249 271 254 9.98 3.9% Sample 3 1068 1033 966 984 1012 40.23 3.9% Sample 3 1068 1033 966 984 1012 40.23 3.9%

Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; GDF15; MIC1, PDF, PLAB, PTGFB

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 252 1068 53 266 1115 Standard deviation 3.55 16.12 74.76 3.92 20.21 81.39 CV (%) 6.7% 6.4% 7% 7.4% 7.6% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 252 1068 53 266 1115 Standard deviation 3.55 16.12 74.76 3.92 20.21 81.39 CV (%) 6.7% 6.4% 7% 7.4% 7.6% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 252 1068 53 266 1115 Mean (pg/ml) 53 252 1068 53 266 1115 Standard deviation 3.55 16.12 74.76 3.92 20.21 81.39 Standard deviation 3.55 16.12 74.76 3.92 20.21 81.39 CV (%) 6.7% 6.4% 7% 7.4% 7.6% 7.3% CV (%) 6.7% 6.4% 7% 7.4% 7.6% 7.3%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.136 0.309 0.480 0.849 1.352 1.522 2.066 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.136 0.309 0.480 0.849 1.352 1.522 2.066 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.136 0.309 0.480 0.849 1.352 1.522 2.066 O.D. 0.002 0.136 0.309 0.480 0.849 1.352 1.522 2.066

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.