Human CD25/IL-2sR Alpha PicoKine® Quick ELISA Kit

Human CD25/IL-2sR Alpha PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human IL2RA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 116 139 115 127 12.01 9.4% Sample 2 719 735 616 709 694 46.4 6.6% Sample 3 2353 2484 2499 2400 2434 60.08 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 116 139 115 127 12.01 9.4% Sample 1 140 116 139 115 127 12.01 9.4% Sample 2 719 735 616 709 694 46.4 6.6% Sample 2 719 735 616 709 694 46.4 6.6% Sample 3 2353 2484 2499 2400 2434 60.08 2.4% Sample 3 2353 2484 2499 2400 2434 60.08 2.4%

Synonyms

Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA

Gene Name

IL2RA

Gene ID

3559

UniProt

P01589

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-C213

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2%

Sensitivity

<5 pg/mL

Function

Receptor for interleukin-2.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30819 MW

Specificity

Natural and recombinant human CD25

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-2 receptor subunit alpha

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 2 Sushi (CCP/SCR) domains.

Protein Name

Interleukin-2 receptor subunit alpha

Sample Data

Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.