Human sL-Selectin PicoKine® Quick ELISA Kit

Human sL-Selectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human SELL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 209 190 190 196 7.77 3.9% Sample 2 543 572 564 574 563 12.27 2.1% Sample 3 1239 1413 1184 1268 1276 84.65 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 209 190 190 196 7.77 3.9% Sample 1 195 209 190 190 196 7.77 3.9% Sample 2 543 572 564 574 563 12.27 2.1% Sample 2 543 572 564 574 563 12.27 2.1% Sample 3 1239 1413 1184 1268 1276 84.65 6.6% Sample 3 1239 1413 1184 1268 1276 84.65 6.6%

Synonyms

L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD62L; SELL; LNHR, LYAM1

Gene Name

SELL

Gene ID

6402

UniProt

P14151

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W39-N332

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in B-cell lines and T-lymphocytes. .

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5%

Sensitivity

<5 pg/mL

Function

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

42187 MW

Specificity

Natural and recombinant Human SELL

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

L-selectin

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the selectin/LECAM family.

Protein Name

L-selectin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.262 0.446 0.852 1.492 2.205 2.476 2.617 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.262 0.446 0.852 1.492 2.205 2.476 2.617 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.262 0.446 0.852 1.492 2.205 2.476 2.617 O.D. 0.011 0.262 0.446 0.852 1.492 2.205 2.476 2.617

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.