Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 185 185 168 174 11.21 6.4% Sample 2 1404 1352 1232 1273 1315 66.97 5% Sample 3 4588 4716 4423 4674 4600 112.25 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 185 185 168 174 11.21 6.4% Sample 1 159 185 185 168 174 11.21 6.4% Sample 2 1404 1352 1232 1273 1315 66.97 5% Sample 2 1404 1352 1232 1273 1315 66.97 5% Sample 3 4588 4716 4423 4674 4600 112.25 2.4% Sample 3 4588 4716 4423 4674 4600 112.25 2.4%

Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st

Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 1404 4588 158 1473 4382 Standard deviation 11.6 71.6 339.51 14.06 81.01 333.03 CV (%) 7.3% 7.6%% 7.4% 8.9% 5.5% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 1404 4588 158 1473 4382 Standard deviation 11.6 71.6 339.51 14.06 81.01 333.03 CV (%) 7.3% 7.6%% 7.4% 8.9% 5.5% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 159 1404 4588 158 1473 4382 Mean (pg/ml) 159 1404 4588 158 1473 4382 Standard deviation 11.6 71.6 339.51 14.06 81.01 333.03 Standard deviation 11.6 71.6 339.51 14.06 81.01 333.03 CV (%) 7.3% 7.6%% 7.4% 8.9% 5.5% 7.6% CV (%) 7.3% 7.6%% 7.4% 8.9% 5.5% 7.6%

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.088 0.133 0.199 0.307 0.484 0.829 1.254 1.911 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.088 0.133 0.199 0.307 0.484 0.829 1.254 1.911 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.088 0.133 0.199 0.307 0.484 0.829 1.254 1.911 O.D. 0.088 0.133 0.199 0.307 0.484 0.829 1.254 1.911

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.