Human TNFRSF14/HVEM PicoKine® Quick ELISA Kit

Human TNFRSF14/HVEM PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human TNFRSF14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a biional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 32 33 31 32 0.82 2.5% Sample 2 202 207 234 232 218 14.37 6.5% Sample 3 389 428 413 386 404 17.36 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 32 33 31 32 0.82 2.5% Sample 1 33 32 33 31 32 0.82 2.5% Sample 2 202 207 234 232 218 14.37 6.5% Sample 2 202 207 234 232 218 14.37 6.5% Sample 3 389 428 413 386 404 17.36 4.2% Sample 3 389 428 413 386 404 17.36 4.2%

Synonyms

Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; Tumor necrosis factor receptor-like 2; TR2; CD270; TNFRSF14; HVEA, HVEM; UNQ329/PRO509

Gene Name

TNFRSF14

Gene ID

8764

UniProt

Q92956

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L39-V202

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Widely expressed, with the highest expression in lung, spleen and thymus.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 202 389 32 205 420 Standard deviation 1.58 5.8%8 23.34 1.85 11.89 27.3 CV (%) 4.8% 4.1% 6% 5.8% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 202 389 32 205 420 Standard deviation 1.58 5.8%8 23.34 1.85 11.89 27.3 CV (%) 4.8% 4.1% 6% 5.8% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 202 389 32 205 420 Mean (pg/ml) 33 202 389 32 205 420 Standard deviation 1.58 5.8%8 23.34 1.85 11.89 27.3 Standard deviation 1.58 5.8%8 23.34 1.85 11.89 27.3 CV (%) 4.8% 4.1% 6% 5.8% 5.8% 6.5% CV (%) 4.8% 4.1% 6% 5.8% 5.8% 6.5%

Sensitivity

<1 pg/mL

Function

Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30392 MW

Specificity

Natural and recombinant human TNFRSF14

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 14

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Sequence Similarities

Contains 3 TNFR-Cys repeats.

Protein Name

Protein Tnfrsf14

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.092 0.148 0.267 0.477 0.910 1.518 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.092 0.148 0.267 0.477 0.910 1.518 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.092 0.148 0.267 0.477 0.910 1.518 2.294 O.D. 0.029 0.092 0.148 0.267 0.477 0.910 1.518 2.294

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.