Human TNFRSF18/GITR PicoKine® Quick ELISA Kit

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 136 127 144 135 6.02 4.4% Sample 2 705 808 735 671 729 50.53 6.9% Sample 3 1313 1243 1354 1475 1346 84.26 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 136 127 144 135 6.02 4.4% Sample 1 135 136 127 144 135 6.02 4.4% Sample 2 705 808 735 671 729 50.53 6.9% Sample 2 705 808 735 671 729 50.53 6.9% Sample 3 1313 1243 1354 1475 1346 84.26 6.2% Sample 3 1313 1243 1354 1475 1346 84.26 6.2%

Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD357; TNFRSF18; AITR, GITR; UNQ319/PRO364

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8%

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.095 0.175 0.299 0.596 1.008 1.651 2.111 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.095 0.175 0.299 0.596 1.008 1.651 2.111 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.095 0.175 0.299 0.596 1.008 1.651 2.111 O.D. 0.031 0.095 0.175 0.299 0.596 1.008 1.651 2.111

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.