Mouse TFPI PicoKine® Quick ELISA Kit

Mouse TFPI PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Mouse Tfpi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TFPI (Tissue factor pathway inhibitor), also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 324 315 312 315 5.35 1.6% Sample 2 1506 1410 1529 1285 1432 96.14 6.7% Sample 3 4359 4343 4246 3938 4221 169.29 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 324 315 312 315 5.35 1.6% Sample 1 310 324 315 312 315 5.35 1.6% Sample 2 1506 1410 1529 1285 1432 96.14 6.7% Sample 2 1506 1410 1529 1285 1432 96.14 6.7% Sample 3 4359 4343 4246 3938 4221 169.29 4% Sample 3 4359 4343 4246 3938 4221 169.29 4%

Synonyms

Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI; Tfpi

Gene Name

Tfpi

Gene ID

21788

UniProt

O54819

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-N306

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform alpha is expressed in heart and spleen; isoform beta in heart and lung.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1506 4359 330 1630 4238 Standard deviation 20.15 99.39 217.95 22.44 123.88 216.13 CV (%) 6.5% 6.6% 5% 6.8% 7.6% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1506 4359 330 1630 4238 Standard deviation 20.15 99.39 217.95 22.44 123.88 216.13 CV (%) 6.5% 6.6% 5% 6.8% 7.6% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1506 4359 330 1630 4238 Mean (pg/ml) 310 1506 4359 330 1630 4238 Standard deviation 20.15 99.39 217.95 22.44 123.88 216.13 Standard deviation 20.15 99.39 217.95 22.44 123.88 216.13 CV (%) 6.5% 6.6% 5% 6.8% 7.6% 5.1% CV (%) 6.5% 6.6% 5% 6.8% 7.6% 5.1%

Sensitivity

<10 pg/mL

Function

Inhibits factor X (X (a) ) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34987 MW

Specificity

Natural and recombinant mouse TFPI

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tissue factor pathway inhibitor

Subcellular Location

Secreted.

Protein Name

Tissue factor pathway inhibitor

Sample Data

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10,000 O.D. 0.019 0.068 0.104 0.219 0.380 0.769 1.299 1.957 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10,000 O.D. 0.019 0.068 0.104 0.219 0.380 0.769 1.299 1.957 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10,000 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10,000 O.D. 0.019 0.068 0.104 0.219 0.380 0.769 1.299 1.957 O.D. 0.019 0.068 0.104 0.219 0.380 0.769 1.299 1.957

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.