Rat MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit

Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 18 16 17 0.86 5% Sample 2 54 52 47 47 50 3.08 6.1% Sample 3 267 269 269 304 277 15.46 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 18 16 17 0.86 5% Sample 1 18 18 18 16 17 0.86 5% Sample 2 54 52 47 47 50 3.08 6.1% Sample 2 54 52 47 47 50 3.08 6.1% Sample 3 267 269 269 304 277 15.46 5.5% Sample 3 267 269 269 304 277 15.46 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% CV (%) 6% 6.7% 5.9% 6.2% 8% 6%

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.