Rat MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit

Rat MIP-1Alpha/CCL3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 18 16 17 0.86 5% Sample 2 54 52 47 47 50 3.08 6.1% Sample 3 267 269 269 304 277 15.46 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 18 16 17 0.86 5% Sample 1 18 18 18 16 17 0.86 5% Sample 2 54 52 47 47 50 3.08 6.1% Sample 2 54 52 47 47 50 3.08 6.1% Sample 3 267 269 269 304 277 15.46 5.5% Sample 3 267 269 269 304 277 15.46 5.5%

Synonyms

C-C motif chemokine 3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3; Ccl3; Mip1a, Scya3

Gene Name

Ccl3

Gene ID

25542

UniProt

P50229

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 267 18 56 248 Mean (pg/ml) 18 54 267 18 56 248 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 Standard deviation 1.08 3.61 15.75 1.11 4.48 14.88 CV (%) 6% 6.7% 5.9% 6.2% 8% 6% CV (%) 6% 6.7% 5.9% 6.2% 8% 6%

Sensitivity

<1 pg/mL

Function

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

10335 MW

Specificity

Natural and recombinant rat Ccl3

Anticoagulant

Heparin, EDTA or Citrate

Gene Name Synonym

C-C motif chemokine 3

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 3

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369 O.D. 0.021 0.125 0.220 0.377 0.710 1.249 1.954 2.369

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.