Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

Human CD105 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Sample 3 4821 5271 4779 5474 5086 295.48 5.8%

Synonyms

Endoglin; CD105; ENG; END

Gene Name

ENG

Gene ID

2022

UniProt

P17813

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E26-G586

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Endoglin is restricted to endothelial cells in all tissues except bone marrow.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8%

Sensitivity

<15 pg/mL

Function

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

70578 MW

Specificity

Natural and recombinant human CD105

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Endoglin

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Endoglin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.