Rat CD44 PicoKine® Quick ELISA Kit

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 481 494 514 475 491 14.94 3% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 481 494 514 475 491 14.94 3% Sample 1 481 494 514 475 491 14.94 3% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 2 1665 1566 1588 1645 1616 40.39 2.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4% Sample 3 7855 9238 8886 9256 8808 570.06 6.4%

CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD44; Cd44

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 481 1665 7855 452 1537 7710 Mean (pg/ml) 481 1665 7855 452 1537 7710 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 Standard deviation 28.37 129.87 439.88 32.54 136.79 516.57 CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7% CV (%) 5.6% 7.8% 5.6% 7.2% 8.9% 6.7%

Receptor for hyaluronic acid (HA) . Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254 O.D. 0.035 0.163 0.274 0.458 0.861 1.249 1.832 2.254

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.