Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 12 11 11 0.43 3.9% Sample 2 77 67 78 82 76 5.52 7.2% Sample 3 272 286 279 302 284 11.12 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 12 11 11 0.43 3.9% Sample 1 12 12 12 11 11 0.43 3.9% Sample 2 77 67 78 82 76 5.52 7.2% Sample 2 77 67 78 82 76 5.52 7.2% Sample 3 272 286 279 302 284 11.12 3.9% Sample 3 272 286 279 302 284 11.12 3.9%

C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; MIP-1-gamma; Macrophage inflammatory protein-related protein 2; MRP-2; Small-inducible cytokine A9; CCL9 (29-101) ; CCL9 (30-101) ; CCL9 (31-101) ; Ccl9; Mrp2, Scya10, Scya9

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7%

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.