Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit

Mouse CCL9/Mip 1 Gamma PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Ccl9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 12 11 11 0.43 3.9% Sample 2 77 67 78 82 76 5.52 7.2% Sample 3 272 286 279 302 284 11.12 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 12 12 11 11 0.43 3.9% Sample 1 12 12 12 11 11 0.43 3.9% Sample 2 77 67 78 82 76 5.52 7.2% Sample 2 77 67 78 82 76 5.52 7.2% Sample 3 272 286 279 302 284 11.12 3.9% Sample 3 272 286 279 302 284 11.12 3.9%

Synonyms

C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; MIP-1-gamma; Macrophage inflammatory protein-related protein 2; MRP-2; Small-inducible cytokine A9; CCL9 (29-101) ; CCL9 (30-101) ; CCL9 (31-101) ; Ccl9; Mrp2, Scya10, Scya9

Gene Name

Ccl9

Gene ID

20308

UniProt

P51670

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q22-Q122

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 77 272 12 74 290 Mean (pg/ml) 12 77 272 12 74 290 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 Standard deviation 0.86 4.69 19.04 0.9 4.88 22.33 CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7% CV (%) 7.2% 6.1% 7% 7.5% 6.6% 7.7%

Sensitivity

<1 pg/mL

Function

Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

13871 MW

Specificity

Natural and recombinant mouse CCL9

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 9

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 9

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183 O.D. 0.064 0.138 0.189 0.295 0.476 0.821 1.396 2.183

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.