Human Ficolin-3 PicoKine® Quick ELISA Kit

Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1216 1347 1267 1181 1252 62.41 4.9% Sample 2 5741 5533 5876 6484 5908 354.01 5.9% Sample 3 26480 26566 26529 22150 25431 1894.67 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1216 1347 1267 1181 1252 62.41 4.9% Sample 1 1216 1347 1267 1181 1252 62.41 4.9% Sample 2 5741 5533 5876 6484 5908 354.01 5.9% Sample 2 5741 5533 5876 6484 5908 354.01 5.9% Sample 3 26480 26566 26529 22150 25431 1894.67 7.4% Sample 3 26480 26566 26529 22150 25431 1894.67 7.4%

Liver and lung. In liver it is produced by bile duct epithelial cells and hepatocytes. In lung it is produced by both ciliated bronchial epithelial cells and type II alveolar epithelial cells. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1216 5741 26480 1219 6253 25739 Standard deviation 80.25 396.12 1403.44 80.45 450.21 1853.2 CV (%) 6.6% 6.9% 5.3% 6.6% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1216 5741 26480 1219 6253 25739 Standard deviation 80.25 396.12 1403.44 80.45 450.21 1853.2 CV (%) 6.6% 6.9% 5.3% 6.6% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1216 5741 26480 1219 6253 25739 Mean (pg/ml) 1216 5741 26480 1219 6253 25739 Standard deviation 80.25 396.12 1403.44 80.45 450.21 1853.2 Standard deviation 80.25 396.12 1403.44 80.45 450.21 1853.2 CV (%) 6.6% 6.9% 5.3% 6.6% 7.2% 7.2% CV (%) 6.6% 6.9% 5.3% 6.6% 7.2% 7.2%

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.159 0.188 0.239 0.323 0.494 0.817 1.264 1.915 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.159 0.188 0.239 0.323 0.494 0.817 1.264 1.915 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.159 0.188 0.239 0.323 0.494 0.817 1.264 1.915 O.D. 0.159 0.188 0.239 0.323 0.494 0.817 1.264 1.915

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.