Human M-CSF ELISA Kit PicoKine®

M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 153 143 153 150 4.2 2.8% Sample 2 681 697 651 751 695 36.3 5.2% Sample 3 1139 1296 1284 1344 1265 76.54 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 153 143 153 150 4.2 2.8% Sample 1 152 153 143 153 150 4.2 2.8% Sample 2 681 697 651 751 695 36.3 5.2% Sample 2 681 697 651 751 695 36.3 5.2% Sample 3 1139 1296 1284 1344 1265 76.54 6% Sample 3 1139 1296 1284 1344 1265 76.54 6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV (%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV (%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 152 681 1139 140 716 1136 Mean (pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV (%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6% CV (%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6%

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.