Human CCL22/MDC PicoKine® Quick ELISA Kit

Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22) . MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 96 88 94 93 3.49 3.7% Sample 2 212 195 228 218 213 11.98 5.6% Sample 3 842 819 834 802 824 15.27 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 96 88 94 93 3.49 3.7% Sample 1 97 96 88 94 93 3.49 3.7% Sample 2 212 195 228 218 213 11.98 5.6% Sample 2 212 195 228 218 213 11.98 5.6% Sample 3 842 819 834 802 824 15.27 1.8% Sample 3 842 819 834 802 824 15.27 1.8%

C-C motif chemokine 22; CC chemokine STCP-1; MDC (1-69) ; Macrophage-derived chemokine; Small-inducible cytokine A22; Stimulated T-cell chemotactic protein 1; MDC (3-69) ; MDC (5-69) ; MDC (7-69) ; CCL22; MDC, SCYA22; A-152E5.1

Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+) . Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 212 842 87 210 866 Standard deviation 7.37 9.11 55.57 7.91 12.6 64.08 CV (%) 7.6% 4.3% 6.6% 9.1% 6% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 212 842 87 210 866 Standard deviation 7.37 9.11 55.57 7.91 12.6 64.08 CV (%) 7.6% 4.3% 6.6% 9.1% 6% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 212 842 87 210 866 Mean (pg/ml) 97 212 842 87 210 866 Standard deviation 7.37 9.11 55.57 7.91 12.6 64.08 Standard deviation 7.37 9.11 55.57 7.91 12.6 64.08 CV (%) 7.6% 4.3% 6.6% 9.1% 6% 7.4% CV (%) 7.6% 4.3% 6.6% 9.1% 6% 7.4%

May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC (3-69), MDC (5-69) and MDC (7-69) seem not be active.

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.101 0.162 0.207 0.301 0.468 0.791 1.345 2.101 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.101 0.162 0.207 0.301 0.468 0.791 1.345 2.101 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.101 0.162 0.207 0.301 0.468 0.791 1.345 2.101 O.D. 0.101 0.162 0.207 0.301 0.468 0.791 1.345 2.101

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.