Human RBP4/Retinol Binding Protein 4 PicoKine® Quick ELISA Kit

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 621 574 637 603 608 23.39 3.8% Sample 2 2874 2703 2656 2896 2782 104.37 3.7% Sample 3 9419 8739 8492 8429 8769 392.34 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 621 574 637 603 608 23.39 3.8% Sample 1 621 574 637 603 608 23.39 3.8% Sample 2 2874 2703 2656 2896 2782 104.37 3.7% Sample 2 2874 2703 2656 2896 2782 104.37 3.7% Sample 3 9419 8739 8492 8429 8769 392.34 4.4% Sample 3 9419 8739 8492 8429 8769 392.34 4.4%

Retinol-binding protein 4; Plasma retinol-binding protein; PRBP; RBP; Plasma retinol-binding protein (1-182) ; Plasma retinol-binding protein (1-181) ; Plasma retinol-binding protein (1-179) ; Plasma retinol-binding protein (1-176) ; RBP4; PRO2222

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1% 7.9% 7.1% 8.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1% 7.9% 7.1% 8.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 621 2874 9419 643 2778 10172 Mean (pg/ml) 621 2874 9419 643 2778 10172 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 Standard deviation 44.09 227.04 668.74 53.36 247.24 752.72 CV (%) 7.1% 7.9% 7.1% 8.3% 8.9% 7.4% CV (%) 7.1% 7.9% 7.1% 8.3% 8.9% 7.4%

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 200000000 20000000 2000000 200000 20000 O.D. 0.029 0.066 0.108 0.215 0.495 1.074 1.866 2.408 Concentration (pg/ml) 0 312 625 200000000 20000000 2000000 200000 20000 O.D. 0.029 0.066 0.108 0.215 0.495 1.074 1.866 2.408 Concentration (pg/ml) 0 312 625 200000000 20000000 2000000 200000 20000 Concentration (pg/ml) 0 312 625 200000000 20000000 2000000 200000 20000 O.D. 0.029 0.066 0.108 0.215 0.495 1.074 1.866 2.408 O.D. 0.029 0.066 0.108 0.215 0.495 1.074 1.866 2.408

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.