Human soluble CD6 PicoKine® Quick ELISA Kit

Human soluble CD6 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 255 257 218 221 237 18.29 7.7% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 3 4911 4340 4503 4742 4624 218.85 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 255 257 218 221 237 18.29 7.7% Sample 1 255 257 218 221 237 18.29 7.7% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 3 4911 4340 4503 4742 4624 218.85 4.7% Sample 3 4911 4340 4503 4742 4624 218.85 4.7%

Synonyms

T-cell differentiation antigen CD6; T12; TP120; CD6; CD6

Gene Name

CD6

Gene ID

923

UniProt

P30203

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H18-E398

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5%

Sensitivity

<10 pg/mL

Function

Involved in cell adhesion. Binds to CD166.

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

71801 MW

Specificity

Natural and recombinant human CD6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

T-cell differentiation antigen CD6

Subcellular Location

Cell membrane ; Single-pass type I membrane protein .

Protein Name

T-cell differentiation antigen CD6

Sample Data

Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.310 0.516 0.912 1.535 2.266 2.421 2.460 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.310 0.516 0.912 1.535 2.266 2.421 2.460 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.051 0.310 0.516 0.912 1.535 2.266 2.421 2.460 O.D. 0.051 0.310 0.516 0.912 1.535 2.266 2.421 2.460

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.