Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit

Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the target cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 41 34 38 3.08 8.1% Sample 2 186 197 192 198 193 4.76 2.4% Sample 3 437 495 446 507 471 30.21 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 41 34 38 3.08 8.1% Sample 1 36 41 41 34 38 3.08 8.1% Sample 2 186 197 192 198 193 4.76 2.4% Sample 2 186 197 192 198 193 4.76 2.4% Sample 3 437 495 446 507 471 30.21 6.4% Sample 3 437 495 446 507 471 30.21 6.4%

Tumor necrosis factor ligand superfamily member 6; Apoptosis antigen ligand; APTL; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD178; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL; ADAM10-processed FasL form; APL; FasL intracellular domain; FasL ICD; SPPL2A-processed FasL form; SPA; FASLG; APT1LG1, CD95L, FASL, TNFSF6

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9%

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058) . Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603) . Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity) . TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity) . Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260) . .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (PubMed:17164290) . Colocalizes with the SPPL2A protease at the cell membrane (PubMed:17557115) . .

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.114 0.196 0.379 0.740 1.371 2.121 2.693 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.114 0.196 0.379 0.740 1.371 2.121 2.693 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.114 0.196 0.379 0.740 1.371 2.121 2.693 O.D. 0.008 0.114 0.196 0.379 0.740 1.371 2.121 2.693

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.