Human BMP-2 PicoKine® Quick ELISA Kit

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 58 51 53 2.94 5.5% Sample 2 250 245 240 275 252 13.46 5.3% Sample 3 1005 885 885 952 931 50.36 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 58 51 53 2.94 5.5% Sample 1 55 51 58 51 53 2.94 5.5% Sample 2 250 245 240 275 252 13.46 5.3% Sample 2 250 245 240 275 252 13.46 5.3% Sample 3 1005 885 885 952 931 50.36 5.4% Sample 3 1005 885 885 952 931 50.36 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 250 1005 59 255 1061 Standard deviation 4.29 13.25 70.35 4.66 15.55 83.81 CV (%) 7.8% 5.3% 7% 7.9% 6.1% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 250 1005 59 255 1061 Standard deviation 4.29 13.25 70.35 4.66 15.55 83.81 CV (%) 7.8% 5.3% 7% 7.9% 6.1% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 250 1005 59 255 1061 Mean (pg/ml) 55 250 1005 59 255 1061 Standard deviation 4.29 13.25 70.35 4.66 15.55 83.81 Standard deviation 4.29 13.25 70.35 4.66 15.55 83.81 CV (%) 7.8% 5.3% 7% 7.9% 6.1% 7.9% CV (%) 7.8% 5.3% 7% 7.9% 6.1% 7.9%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.089 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.089 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.089 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.089

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.