Human IL-1 RL1 / ST2 PicoKine® Quick ELISA Kit

Human IL-1 RL1 / ST2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 253 262 247 251 7.44 2.9% Sample 2 1795 1679 1561 1563 1649 96.63 5.8% Sample 3 5486 5339 5211 5406 5360 100.78 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 253 262 247 251 7.44 2.9% Sample 1 242 253 262 247 251 7.44 2.9% Sample 2 1795 1679 1561 1563 1649 96.63 5.8% Sample 2 1795 1679 1561 1563 1649 96.63 5.8% Sample 3 5486 5339 5211 5406 5360 100.78 1.8% Sample 3 5486 5339 5211 5406 5360 100.78 1.8%

Synonyms

Interleukin-1 receptor-like 1; Protein ST2; IL1RL1; DER4, ST2, T1

Gene Name

IL1RL1

Gene ID

9173

UniProt

Q01638

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K19-S328

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. .

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1795 5486 247 1778 5061 Standard deviation 9.68 80.77 257.84 13.58 106.68 237.86 CV (%) 4% 4.5% 4.7% 5.5% 6% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1795 5486 247 1778 5061 Standard deviation 9.68 80.77 257.84 13.58 106.68 237.86 CV (%) 4% 4.5% 4.7% 5.5% 6% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1795 5486 247 1778 5061 Mean (pg/ml) 242 1795 5486 247 1778 5061 Standard deviation 9.68 80.77 257.84 13.58 106.68 237.86 Standard deviation 9.68 80.77 257.84 13.58 106.68 237.86 CV (%) 4% 4.5% 4.7% 5.5% 6% 4.7% CV (%) 4% 4.5% 4.7% 5.5% 6% 4.7%

Sensitivity

<10 pg/mL

Function

Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

63358 MW

Specificity

Natural and recombinant human IL1RL1 as well as free IL1RL1 and IL-33 complexed IL1RL1.

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-1 receptor-like 1

Subcellular Location

Isoform C: Cell membrane.

Sequence Similarities

Belongs to the interleukin-1 receptor family.

Protein Name

Interleukin-1 receptor-like 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.081 0.135 0.223 0.411 0.834 1.425 1.979 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.081 0.135 0.223 0.411 0.834 1.425 1.979 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.081 0.135 0.223 0.411 0.834 1.425 1.979 O.D. 0.014 0.081 0.135 0.223 0.411 0.834 1.425 1.979

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.