Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit

Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. Whata€?s more, EPO is also involved in the wound healing process.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 117 110 115 112 3.64 3.2% Sample 2 342 374 386 373 368 16.26 4.4% Sample 3 1332 1189 1227 1391 1284 80.66 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 117 110 115 112 3.64 3.2% Sample 1 108 117 110 115 112 3.64 3.2% Sample 2 342 374 386 373 368 16.26 4.4% Sample 2 342 374 386 373 368 16.26 4.4% Sample 3 1332 1189 1227 1391 1284 80.66 6.2% Sample 3 1332 1189 1227 1391 1284 80.66 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 342 1332 113 322 1373 Standard deviation 5.4 25.3%9 70.59 6.66 30.26 86.49 CV (%) 5% 7.6% 5.3% 5.9% 9.4% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 342 1332 113 322 1373 Standard deviation 5.4 25.3%9 70.59 6.66 30.26 86.49 CV (%) 5% 7.6% 5.3% 5.9% 9.4% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 342 1332 113 322 1373 Mean (pg/ml) 108 342 1332 113 322 1373 Standard deviation 5.4 25.3%9 70.59 6.66 30.26 86.49 Standard deviation 5.4 25.3%9 70.59 6.66 30.26 86.49 CV (%) 5% 7.6% 5.3% 5.9% 9.4% 6.3% CV (%) 5% 7.6% 5.3% 5.9% 9.4% 6.3%

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.000 0.008 0.016 0.066 0.162 0.449 1.420 2.290 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.000 0.008 0.016 0.066 0.162 0.449 1.420 2.290 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.000 0.008 0.016 0.066 0.162 0.449 1.420 2.290 O.D. 0.000 0.008 0.016 0.066 0.162 0.449 1.420 2.290

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.