Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit

Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF) . IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 10 10 9 9 0.5 5.5% Sample 2 73 73 74 62 70 4.92 7% Sample 3 238 246 219 235 234 9.81 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 10 10 9 9 0.5 5.5% Sample 1 9 10 10 9 9 0.5 5.5% Sample 2 73 73 74 62 70 4.92 7% Sample 2 73 73 74 62 70 4.92 7% Sample 3 238 246 219 235 234 9.81 4.1% Sample 3 238 246 219 235 234 9.81 4.1%

Synonyms

Interleukin-5; IL-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; IL5

Gene Name

IL5

Gene ID

3567

UniProt

P05113

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I20-S134

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 9 73 238 8 70 234 Standard deviation 0.7 4.67 16.42 0.74 4.55 20.12 CV (%) 7.8% 6.4% 6.9% 9.3% 6.5% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 9 73 238 8 70 234 Standard deviation 0.7 4.67 16.42 0.74 4.55 20.12 CV (%) 7.8% 6.4% 6.9% 9.3% 6.5% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 9 73 238 8 70 234 Mean (pg/ml) 9 73 238 8 70 234 Standard deviation 0.7 4.67 16.42 0.74 4.55 20.12 Standard deviation 0.7 4.67 16.42 0.74 4.55 20.12 CV (%) 7.8% 6.4% 6.9% 9.3% 6.5% 8.6% CV (%) 7.8% 6.4% 6.9% 9.3% 6.5% 8.6%

Sensitivity

<2 pg/mL

Function

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15238 MW

Specificity

Natural and recombinant human IL-5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-5

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-5 family.

Protein Name

Interleukin-5

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.005 0.078 0.150 0.269 0.501 0.862 1.545 2.201 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.005 0.078 0.150 0.269 0.501 0.862 1.545 2.201 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.005 0.078 0.150 0.269 0.501 0.862 1.545 2.201 O.D. 0.005 0.078 0.150 0.269 0.501 0.862 1.545 2.201

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.