Human TGFBR3/Tgf Beta Riii PicoKine® Quick ELISA Kit

Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved ly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 199 223 196 201 13.58 6.7% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 3 5554 6224 5744 5545 5766 275.69 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 199 223 196 201 13.58 6.7% Sample 1 186 199 223 196 201 13.58 6.7% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 3 5554 6224 5744 5545 5766 275.69 4.7% Sample 3 5554 6224 5744 5545 5766 275.69 4.7%

Transforming growth factor beta receptor type 3; TGF-beta receptor type 3; TGFR-3; Betaglycan; Transforming growth factor beta receptor III; TGF-beta receptor type III; TGFBR3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. Secreted, extracellular space. Cell membrane ; Single-pass type I membrane protein . Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.002 0.070 0.125 0.269 0.523 0.943 1.529 2.132 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.002 0.070 0.125 0.269 0.523 0.943 1.529 2.132 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.002 0.070 0.125 0.269 0.523 0.943 1.529 2.132 O.D. 0.002 0.070 0.125 0.269 0.523 0.943 1.529 2.132

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.