Human CD163/M130 PicoKine® Quick ELISA Kit

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3520 3403 3222 3050 3298 178.59 5.4% Sample 2 11456 11119 13238 12078 11972 807.44 6.7% Sample 3 44674 42932 42302 44756 43666 1072.77 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3520 3403 3222 3050 3298 178.59 5.4% Sample 1 3520 3403 3222 3050 3298 178.59 5.4% Sample 2 11456 11119 13238 12078 11972 807.44 6.7% Sample 2 11456 11119 13238 12078 11972 807.44 6.7% Sample 3 44674 42932 42302 44756 43666 1072.77 2.4% Sample 3 44674 42932 42302 44756 43666 1072.77 2.4%

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 CV (%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 CV (%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 CV (%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9% CV (%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9%

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.011 0.255 0.405 0.794 1.291 1.820 2.205 2.478 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.011 0.255 0.405 0.794 1.291 1.820 2.205 2.478 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.011 0.255 0.405 0.794 1.291 1.820 2.205 2.478 O.D. 0.011 0.255 0.405 0.794 1.291 1.820 2.205 2.478

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.