Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit

Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Mouse Tnfrsf11b in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 157 157 146 148 9.88 6.6% Sample 2 800 766 743 710 754 32.84 4.3% Sample 3 3535 3949 3581 3584 3662 166.69 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 157 157 146 148 9.88 6.6% Sample 1 133 157 157 146 148 9.88 6.6% Sample 2 800 766 743 710 754 32.84 4.3% Sample 2 800 766 743 710 754 32.84 4.3% Sample 3 3535 3949 3581 3584 3662 166.69 4.5% Sample 3 3535 3949 3581 3584 3662 166.69 4.5%

Synonyms

Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; Tnfrsf11b; Ocif, Opg

Gene Name

Tnfrsf11b

Gene ID

18383

UniProt

O08712

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-L401

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.3%9 55.3%9 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.3%9 55.3%9 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 800 3535 139 767 3643 Mean (pg/ml) 133 800 3535 139 767 3643 Standard deviation 5.85 54.4 187.35 7.3%9 55.3%9 225.86 Standard deviation 5.85 54.4 187.35 7.3%9 55.3%9 225.86 CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2% CV (%) 4.4% 6.8% 5.3% 4.6% 7.3% 6.2%

Sensitivity

<5 pg/mL

Function

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

45923 MW

Specificity

Natural and recombinant mouse OPG

Anticoagulant

EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 11B

Subcellular Location

Secreted.

Sequence Similarities

Contains 2 death domains.

Protein Name

Tumor necrosis factor receptor superfamily member 11B

Sample Data

Concentration (pg/ml) 0 93.7 187.5 375 750 13000 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.118 Concentration (pg/ml) 0 93.7 187.5 375 750 13000 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.118 Concentration (pg/ml) 0 93.7 187.5 375 750 13000 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 13000 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.118 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.118

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.