Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 32 35 35 2.12 6% Sample 2 143 161 140 157 150 8.92 5.9% Sample 3 444 418 452 413 431 16.58 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 32 35 35 2.12 6% Sample 1 38 35 32 35 35 2.12 6% Sample 2 143 161 140 157 150 8.92 5.9% Sample 2 143 161 140 157 150 8.92 5.9% Sample 3 444 418 452 413 431 16.58 3.8% Sample 3 444 418 452 413 431 16.58 3.8%

Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) . Levels rapidly diminish as soon as ESC's differentiate (at protein levels) . Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 143 444 35 150 444 Mean (pg/ml) 38 143 444 35 150 444 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 Standard deviation 1.63 6 33.74 1.82 7.5 37.29 CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4% CV (%) 4.3% 4.2% 7.6% 5.2% 5% 8.4%

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.288 0.433 0.647 1.088 1.501 2.024 2.689 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.288 0.433 0.647 1.088 1.501 2.024 2.689 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.288 0.433 0.647 1.088 1.501 2.024 2.689 O.D. 0.024 0.288 0.433 0.647 1.088 1.501 2.024 2.689

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.