Human TIMP-2 PicoKine® Quick ELISA Kit

Human TIMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Sample 3 4399 5066 4506 5016 4746 297.19 6.2%

Synonyms

Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2; TIMP2

Gene Name

TIMP2

Gene ID

7077

UniProt

P16035

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: C27-P220

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.4%3 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.4%3 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.4%3 94.65 325.52 16.73 112.47 361.54 Standard deviation 17.4%3 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3%

Sensitivity

<2 pg/mL

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24399 MW

Specificity

Natural and recombinant human TIMP-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Metalloproteinase inhibitor 2

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Protein Name

Metalloproteinase inhibitor 2

Sample Data

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.005 0.109 0.198 0.418 0.787 1.397 2.109 2.513 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.005 0.109 0.198 0.418 0.787 1.397 2.109 2.513 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.005 0.109 0.198 0.418 0.787 1.397 2.109 2.513 O.D. 0.005 0.109 0.198 0.418 0.787 1.397 2.109 2.513

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.