Human IL-15/Interleukin-15 PicoKine® Quick ELISA Kit

Interleukin (IL) -15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 37 37 35 36 1 2.7% Sample 2 140 151 160 164 153 9.22 6% Sample 3 427 395 364 407 398 22.84 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 37 37 35 36 1 2.7% Sample 1 35 37 37 35 36 1 2.7% Sample 2 140 151 160 164 153 9.22 6% Sample 2 140 151 160 164 153 9.22 6% Sample 3 427 395 364 407 398 22.84 5.7% Sample 3 427 395 364 407 398 22.84 5.7%

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1%

Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.