Mouse VEGF PicoKine® Quick ELISA Kit

Mouse VEGF PicoKine

Product Specifications

Background

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Sample 3 538 463 494 471 491 29.15 5.9%

Synonyms

Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; Vegfa; Vegf

Gene Name

Vegfa

Gene ID

22339

UniProt

Q00731

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R190

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5%

Sensitivity

<2 pg/mL

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity) . .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

25283 MW

Specificity

Natural and recombinant Mouse Vegfa

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Vascular endothelial growth factor A

Subcellular Location

Isoform VEGF-1: Secreted.

Sequence Similarities

Belongs to the PDGF/VEGF growth factor family.

Protein Name

Vascular endothelial growth factor A

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.