Human PLAT/TPA PicoKine® Quick ELISA Kit

Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 72 75 77 77 4.41 5.7% Sample 2 237 271 264 232 251 16.77 6.6% Sample 3 975 906 909 870 915 37.88 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 72 75 77 77 4.41 5.7% Sample 1 84 72 75 77 77 4.41 5.7% Sample 2 237 271 264 232 251 16.77 6.6% Sample 2 237 271 264 232 251 16.77 6.6% Sample 3 975 906 909 870 915 37.88 4.1% Sample 3 975 906 909 870 915 37.88 4.1%

Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA;3.4.21.68; Alteplase; Reteplase; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; PLAT

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 237 975 81 229 878 Standard deviation 5.37 15.4 43.87 5.58 17.63 43.02 CV (%) 6.4% 6.5% 4.5% 6.9% 7.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 237 975 81 229 878 Standard deviation 5.37 15.4 43.87 5.58 17.63 43.02 CV (%) 6.4% 6.5% 4.5% 6.9% 7.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 237 975 81 229 878 Mean (pg/ml) 84 237 975 81 229 878 Standard deviation 5.37 15.4 43.87 5.58 17.63 43.02 Standard deviation 5.37 15.4 43.87 5.58 17.63 43.02 CV (%) 6.4% 6.5% 4.5% 6.9% 7.7% 4.9% CV (%) 6.4% 6.5% 4.5% 6.9% 7.7% 4.9%

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a role in facilitating neuronal migration.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.177 0.29 0.764 1.091 1.82 2.441 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.177 0.29 0.764 1.091 1.82 2.441 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.013 0.093 0.177 0.29 0.764 1.091 1.82 2.441 O.D. 0.013 0.093 0.177 0.29 0.764 1.091 1.82 2.441

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.