Human GM-CSF PicoKine® Quick ELISA Kit

Human GM-CSF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Sample 3 388 374 388 433 395 22.25 5.6%

Synonyms

Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF

Gene Name

CSF2

Gene ID

1437

UniProt

P04141

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A18-E144

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4%

Sensitivity

<1 pg/mL

Function

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

16295 MW

Specificity

Natural and recombinant human CSF2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Granulocyte-macrophage colony-stimulating factor

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the GM-CSF family.

Protein Name

Granulocyte-macrophage colony-stimulating factor

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016 O.D. 0.022 0.233 0.439 0.799 1.215 1.432 1.736 2.016

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.