Mouse GDNF PicoKine® Quick ELISA Kit

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 101 104 97 100 2.48 2.4% Sample 2 227 204 214 227 218 9.66 4.4% Sample 3 789 928 792 891 850 60.93 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 101 104 97 100 2.48 2.4% Sample 1 101 101 104 97 100 2.48 2.4% Sample 2 227 204 214 227 218 9.66 4.4% Sample 2 227 204 214 227 218 9.66 4.4% Sample 3 789 928 792 891 850 60.93 7.1% Sample 3 789 928 792 891 850 60.93 7.1%

Expressed in both the central nervous system (CNS) and in non-CNS tissues. Expressed in a highly dynamic pattern in the anterior neuroectoderm during the early stages of neurogenesis between E7.5 and E10.5. Beginning at E10.5, expression begins in mesenchymal tissues of several organs including the digestive tract, kidney, testis, frontonasal mass, tooth primordium, tongue, mandible, whisker follicles, ear, eye, limb bud and in distinct regions of the brain. Also expressed in the heart, ileum, liver and muscle. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 227 789 101 217 855 Standard deviation 7.57 9.3 57.53 7.67 9.11 66.69 CV (%) 7.5% 4.1% 7% 7.6% 4.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 227 789 101 217 855 Standard deviation 7.57 9.3 57.53 7.67 9.11 66.69 CV (%) 7.5% 4.1% 7% 7.6% 4.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 227 789 101 217 855 Mean (pg/ml) 101 227 789 101 217 855 Standard deviation 7.57 9.3 57.53 7.67 9.11 66.69 Standard deviation 7.57 9.3 57.53 7.67 9.11 66.69 CV (%) 7.5% 4.1% 7% 7.6% 4.2% 7.8% CV (%) 7.5% 4.1% 7% 7.6% 4.2% 7.8%

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.