Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 76 77 70 73 3.53 4.8% Sample 2 264 272 267 262 266 3.76 1.4% Sample 3 855 943 952 812 890 59.07 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 76 77 70 73 3.53 4.8% Sample 1 69 76 77 70 73 3.53 4.8% Sample 2 264 272 267 262 266 3.76 1.4% Sample 2 264 272 267 262 266 3.76 1.4% Sample 3 855 943 952 812 890 59.07 6.6% Sample 3 855 943 952 812 890 59.07 6.6%

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6%

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.114 0.170 0.318 0.635 1.025 1.689 2.185 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.114 0.170 0.318 0.635 1.025 1.689 2.185 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.114 0.170 0.318 0.635 1.025 1.689 2.185 O.D. 0.013 0.114 0.170 0.318 0.635 1.025 1.689 2.185

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.