Mouse Resistin PicoKine® Quick ELISA Kit

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 78 88 84 81 4.49 5.5% Sample 2 245 263 267 252 256 8.72 3.4% Sample 3 929 803 900 906 884 48.28 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 78 88 84 81 4.49 5.5% Sample 1 77 78 88 84 81 4.49 5.5% Sample 2 245 263 267 252 256 8.72 3.4% Sample 2 245 263 267 252 256 8.72 3.4% Sample 3 929 803 900 906 884 48.28 5.4% Sample 3 929 803 900 906 884 48.28 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 245 929 80 252 861 Standard deviation 6.2%2 13.23 57.59 7.28 17.64 69.74 CV (%) 7.7% 5.4% 6.2% 9.1% 7% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 245 929 80 252 861 Standard deviation 6.2%2 13.23 57.59 7.28 17.64 69.74 CV (%) 7.7% 5.4% 6.2% 9.1% 7% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 245 929 80 252 861 Mean (pg/ml) 77 245 929 80 252 861 Standard deviation 6.2%2 13.23 57.59 7.28 17.64 69.74 Standard deviation 6.2%2 13.23 57.59 7.28 17.64 69.74 CV (%) 7.7% 5.4% 6.2% 9.1% 7% 8.1% CV (%) 7.7% 5.4% 6.2% 9.1% 7% 8.1%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.006 0.122 0.245 0.441 0.743 1.101 1.748 2.241 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.006 0.122 0.245 0.441 0.743 1.101 1.748 2.241 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.006 0.122 0.245 0.441 0.743 1.101 1.748 2.241 O.D. 0.006 0.122 0.245 0.441 0.743 1.101 1.748 2.241

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.