Human Resistin PicoKine® Quick ELISA Kit

Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 137 143 146 140 4.43 3.1% Sample 2 639 653 634 737 665 41.72 6.2% Sample 3 2067 2270 2337 2337 2252 110.67 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 137 143 146 140 4.43 3.1% Sample 1 135 137 143 146 140 4.43 3.1% Sample 2 639 653 634 737 665 41.72 6.2% Sample 2 639 653 634 737 665 41.72 6.2% Sample 3 2067 2270 2337 2337 2252 110.67 4.9% Sample 3 2067 2270 2337 2337 2252 110.67 4.9%

Resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; RETN; FIZZ3, HXCP1, RSTN; UNQ407/PRO1199

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 639 2067 142 636 2227 Standard deviation 8.1 40.25 161.22 8.52 46.42 177.83 CV (%) 6% 7.3% 7.8% 6% 7.3% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 639 2067 142 636 2227 Standard deviation 8.1 40.25 161.22 8.52 46.42 177.83 CV (%) 6% 7.3% 7.8% 6% 7.3% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 135 639 2067 142 636 2227 Mean (pg/ml) 135 639 2067 142 636 2227 Standard deviation 8.1 40.25 161.22 8.52 46.42 177.83 Standard deviation 8.1 40.25 161.22 8.52 46.42 177.83 CV (%) 6% 7.3% 7.8% 6% 7.3% 7.9% CV (%) 6% 7.3% 7.8% 6% 7.3% 7.9%

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.013 0.169 0.306 0.483 0.926 1.391 1.920 2.305 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.013 0.169 0.306 0.483 0.926 1.391 1.920 2.305 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.013 0.169 0.306 0.483 0.926 1.391 1.920 2.305 O.D. 0.013 0.169 0.306 0.483 0.926 1.391 1.920 2.305

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.