Rat SCGB1A1/uteroglobin PicoKine® Quick ELISA Kit

Rat SCGB1A1/uteroglobin PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 258 259 253 247 16.03 6.4% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 3 4490 4875 4487 4849 4675 186.97 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 220 258 259 253 247 16.03 6.4% Sample 1 220 258 259 253 247 16.03 6.4% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 2 1610 1483 1380 1552 1506 85.63 5.6% Sample 3 4490 4875 4487 4849 4675 186.97 3.9% Sample 3 4490 4875 4487 4849 4675 186.97 3.9%

Synonyms

Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg

Gene Name

Scgb1a1

Gene ID

25575

UniProt

P17559

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S20-V96

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways) .

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 220 1610 4490 241 1635 4849 Mean (pg/ml) 220 1610 4490 241 1635 4849 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 Standard deviation 8.8 123.97 298.6%4 10.36 140.61 374.3%2 CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8% CV (%) 4% 7.7% 6.6% 4.3% 8.6% 7.8%

Sensitivity

<10 pg/mL

Function

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat SCGB1A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Secretoglobin family 1A member 1

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.042 0.075 0.099 0.154 0.263 0.522 1.135 2.156 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.042 0.075 0.099 0.154 0.263 0.522 1.135 2.156 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.042 0.075 0.099 0.154 0.263 0.522 1.135 2.156 O.D. 0.042 0.075 0.099 0.154 0.263 0.522 1.135 2.156

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.