Human Aggrecan/ACAN PicoKine® Quick ELISA Kit

Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3084 3011 3191 2954 3060 88.56 2.8% Sample 2 13064 11683 11338 13036 12280 779.41 6.3% Sample 3 43102 43347 44289 42847 43396 544.9 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3084 3011 3191 2954 3060 88.56 2.8% Sample 1 3084 3011 3191 2954 3060 88.56 2.8% Sample 2 13064 11683 11338 13036 12280 779.41 6.3% Sample 2 13064 11683 11338 13036 12280 779.41 6.3% Sample 3 43102 43347 44289 42847 43396 544.9 1.2% Sample 3 43102 43347 44289 42847 43396 544.9 1.2%

Aggrecan core protein; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1; Aggrecan core protein 2; ACAN; AGC1, CSPG1, MSK16

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3084 13064 43102 3322 13982 40208 Standard deviation 240.55 679.32 1939.59 275.72 908.83 1809.36 CV (%) 7.8% 5.2% 4.5% 8.3% 6.5% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3084 13064 43102 3322 13982 40208 Standard deviation 240.55 679.32 1939.59 275.72 908.83 1809.36 CV (%) 7.8% 5.2% 4.5% 8.3% 6.5% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3084 13064 43102 3322 13982 40208 Mean (pg/ml) 3084 13064 43102 3322 13982 40208 Standard deviation 240.55 679.32 1939.59 275.72 908.83 1809.36 Standard deviation 240.55 679.32 1939.59 275.72 908.83 1809.36 CV (%) 7.8% 5.2% 4.5% 8.3% 6.5% 4.5% CV (%) 7.8% 5.2% 4.5% 8.3% 6.5% 4.5%

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.052 0.109 0.235 0.512 1.033 1.787 2.427 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.052 0.109 0.235 0.512 1.033 1.787 2.427 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.001 0.052 0.109 0.235 0.512 1.033 1.787 2.427 O.D. 0.001 0.052 0.109 0.235 0.512 1.033 1.787 2.427

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.